Reannotation of the cultivated strawberry genome and establishment of a strawberry genome database

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Reannotation of the F. ananassa genome via our prior pipeline

In this study, we created an updated annotation of the most recent F. ananassa genome (Edger et al.2). The new annotation is named version 1.0.a2 (v1.0.a2). The v1.0.a2 reannotation process incorporated full-length transcript data and extensive RNA-seq data from different tissues and at different developmental stages for AS isoform identification and gene structure updates (Fig. 1). First, we used BRAKER v2.1.519 to generate an initial protein-coding gene annotation. The input data for BRAKER included the following: (i) BRAKER-trained models; (ii) intron hints converted from aligned full-length transcript sequences; (iii) intron hints converted from aligned RNA-seq reads and de novo transcripts; (iv) protein hints generated from mapping UniProt, Arabidopsis Araport11 and F. vesca v4.0.a2 protein sequences; and (v) repeat masked genome data. The SMRT full-length sequences were obtained from pooled strawberry tissues (including dwarf stem, flower, mature leaf, and fruit tissues at six different stages)12. Illumina RNA-Seq libraries were obtained from a series of different tissues of F. ananassa, including flower, petal, root, leaf, stem, and fruit tissues at different developmental stages or under different treatments (Table S1)2,14,15,16,17,18. EVidenceModeler (EVM) software20 was then used to combine the different types of evidence into consensus gene models, including gene models predicted by BRAKER, mapped full-length sequences, protein sequences, de novo sequences, and genome-guided transcripts (Fig. 1). Integrative Genomics Viewer (IGV)21 was used to inspect new annotations across the entire genome and select the optimal gene models by comparisons with the mapped RNA-seq reads; approximately 2000 (1.8%) genes were manually curated. Finally, we obtained the new annotation v1.0.a2, which contained a final set of 108,447 genes (Table S2).

Fig. 1: Annotation workflow for F. ananassa protein-coding genes.

The best gene models generated from full-length transcripts were used for training for BRAKER. RNA-seq datasets were used to construct comprehensive transcriptomes via genome-guided and de novo assemblies. F. vesca v4.0.a2 annotation and UniProt and Arabidopsis protein sequences were also inputted into BRAKER. The red dotted box contains the input evidence for EVidenceModeler. In addition, manual curation was conducted to ensure the accuracy of the annotation

F. ananassa annotation v1.0.a2 contains 108,447 protein-coding genes and 127,701 transcripts (Table 1). The locus IDs and position for the newly modified genes in both versions are listed in Table S3. The gene models (coding DNA sequence regions) were compared between v1.0.a1 and v1.0.a2 in gtf format by Cuffcompare22. The transcripts with a class_code ‘=’ classified by Cuffcompare were defined as the same gene model. As a result, a total of 89,215 genes, 82.63% of those of v1.0.a1 and 82.27% of those of v1.0.a2, were shared between the two genome annotation versions (Fig. 2a). In the v1.0.a2 annotation, 360 novel genes (class_code ‘u’) were added (Table 1), and 6993 genes in v1.0.a1 were removed. Furthermore, the model of 19,174 genes was updated and modified (remaining class_codes). The gene IDs in the previous annotation (v1.0.a1) remained the same in the new v1.0.a2 genome, following the format FaxC_XgXXXXX. The statistics comparing v1.0.a1 and v1.0.a2 are shown in Table 1. A total of 86,622 genes were found to have 3′UTRs and/or 5′UTRs, representing approximately 79.9% of all the annotated genes. The average number of exons per gene increased from 5.4 to 5.7. The new annotation also contains alternatively spliced or alternatively initiated transcripts. A total of 30,298 transcripts from 11,044 genes were found, resulting in an average of 1.2 transcript isoforms per gene at the whole-genome scale. In addition, 17,265 genes were assigned GO terms in v1.0.a2, compared to 16,569 genes in v1.0.a1. A total of 76,925 genes acquired functional annotations in v1.0.a2, 3009 more than the number in v1.0.a1 (Table 1).

Table 1 Summary of the v1.0.a2 annotation.
Fig. 2: Comparison of v1.0.a1 and v1.0.a2 annotations.

a Venn diagram showing the common and unique gene structure of the CDS region between v1.0.a1 and v1.0.a2. b Cumulative AED distribution for the v1.0.a1 and v1.0.a2 annotations. c Cumulative QI distribution for the v1.0.a1 and v1.0.a2 annotations

Evaluation of the v1.0.a2 annotation

MAKER223 was used to measure the consistency of gene loci with the available protein and nucleotide sequence alignments, the process of which was based on the mRNA quality index (QI) and annotation edit distance (AED). Each gene was assigned a QI score between 0 and 1, and a higher QI score suggests a higher proportion of exons that match the transcript alignment. The AED score is also between 0 and 1, where 0 indicates complete consistency with the evidence and where 1 means complete inconsistency with the evidence. The AED distribution is shifted toward lower (better) scores in v1.0.a2 compared with v1.0.a1 (Fig. 2b). In contrast, the cumulative QI distribution shows that the QI is shifted toward higher (better) scores in v1.0.a2 compared with v1.0.a1 (Fig. 2c). Therefore, v1.0.a2 has a higher proportion of gene models supported by the transcript evidence.

We used BUSCO v3.0.224 (embryophyta_odb9 database) to assess the completeness of the v1.0.a1 and v1.0.a2 annotations. BUSCO measures genome assembly and annotation completeness based on a curated set of Plantae lineage-specific single-copy orthologs. With respect to the 1440 conserved genes, v1.0.a2 harbors 97.85% complete BUSCOs, compared to 96.88% harbored by v1.0.a1 (Table 1).

Prediction of gene functions

To update the functional annotations of protein-coding genes in v1.0.a2, each of the predicted protein sequences was searched against the InterPro protein database via InterProScan25. Then, eggNOG mapper26 and PANNZER27 were employed to assign GO categories and KEGG functional annotations for all annotated loci. According to the eggNOG results, 17,265 genes were assigned to a specific GO term, compared to 16,569 genes in v1.0.a1 (Tables 1, S4). In addition, the iTAK pipeline28 was used to detect and classify transcription factors and protein kinases. A total of 5695 transcription factors (TFs) and 1340 transcriptional regulators (TRs) were identified in the v1.0.a2 annotation (Table S5). There were 170 more TF genes in v1.0.a2 than in v1.0.a1. Some transcription factor families acquired more members in v1.0.a2, such as the far-red impaired response 1 (FAR1) family, whose members increased from 41 to 163, the B3 family, whose members increased from 223 to 232, the Trihelix family, whose members increased from 108 to 115, and the MYB family, whose members increased from 414 to 440. Furthermore, there are 3611 protein kinase-encoding genes in v1.0.a2, 20 fewer than the number in v1.0.a1 (Table S5).

Below are several examples illustrating the improved accuracy of the v1.0.a2 annotation. FxaC_5g02370, a homolog of AtMYB68 (AT5g65790) encoding an R2R3-MYB family protein with roles in root development29, has a new translation start site, resulting in a longer ORF (Fig. 3a). Two adjacent FaAPM genes (FxaC_5g03320 and FxaC_5g03321), both of which encode a homolog of AtAPM1 (AT4G33090) that interacts with secreted cell surface and cell wall proline-rich proteins30, were originally annotated as a single gene, with FxaC_5g03321 annotated as the 5′UTR of FxaC_5g03320, in v1.0.a1 (Fig. 3b). In addition, the single FxaC_11g03080 gene in v1.0.a1 is split into two genes (FxaC_11g03080 and FxaC_11g03081) in v1.0.a2; gene FxaC_11g03081 encodes a homolog of AtTOD1 (AT5G46220) acting in turgor pressure regulation in both guard cells and pollen tubes in Arabidopsis31 (Fig. 3c).

Fig. 3: Examples of known genes with improved annotation.

a IGV view of the gene model of FxaC_5g02370 according to the old (blue) and new (yellow) annotations. The blue and yellow lines with gene IDs below represent the whole gene. The blue and yellow lines with mRNA IDs below show the gene structure in detail. The thin bars represent UTRs, the thick bars represent ORFs, and the thin lines represent introns. The arrowheads within the bars indicate transcriptional orientation. b IGV view of the RNA-seq mapped reads for the two adjacent FaAPM genes (FxaC_5g03320 and FxaC_5g03321) in v1.0.a2 that correspond to a single gene in v1.0.a1. c FxaC_11g03080 in v1.0.a1 is split into two genes, FxaC_11g03080 and FxaC_11g03081

Reanalysis of the F. ananassa fruit transcriptomes

Several RNA-seq analyses of F. ananassa fruit development have been reported over the past several years16,17; these analyses have used the F. vesca genome as a reference genome due to the absence of a high-quality F. ananassa genome. In this study, we reanalyzed the transcriptome of the four stages of achenes and receptacles, the green (G), white (W), turning (T), and red (R) stages, with three biological replicates each16. Accurate gene expression profiling based on the new genome assembly and annotation should be helpful for genetic and functional research of strawberry fruit development and serves as a valuable resource. The gene read counts and expression profiles (transcripts per million (TPM)) of all the annotated genes in the achene and receptacle tissues across the four stages are presented in Table S6. A total of 42,624 genes (39.3% out of the 108,447 genes) were expressed at a level higher than 2 TPM in at least one of these tissues. The table also indicates their homologous genes in Arabidopsis and the corresponding gene annotation if available. In addition, we analyzed the global gene expression changes in the achenes and receptacles among the different stages, with the expression of more genes being downregulated than upregulated as ripening progressed (Fig. 4a, Tables S7, S8). In both the achenes and receptacles, the greatest number of DEGs between two consecutive stages was found between the green and white stages, which is consistent with the results of previous studies16. For receptacles, the top three enriched GO terms were alpha-amino acid catabolic process, cellular amino acid catabolic process, and carboxylic acid metabolic process, which are related to active metabolic processes occurring during fruit development.

Fig. 4: Differentially expressed genes (DEGs) during fruit development.

a Number of differentially expressed genes identified in the successive transition from the green (G), white (W), turning (T), and red (R) stages in the receptacles (Rs) and in achene (As). b Average profiles of twelve gene clusters of genes in the receptacle. Line plot showing the expression of genes in the twelve gene clusters. The number of genes in each cluster is shown. The Y-axis represents the z-score obtained from the expression level (TPM). c Heatmap of F. ananassa CRU1 genes expressed in different tissues. GR Green receptacle, WR White receptacle, TR Turning receptacle, RR Red receptacle, GA Green achene, WA White achene, TA Turning achene, RA Red achene

To identify the transcriptional dynamics associated with fruit ripening, the DEGs with different expression profiles were assigned to different gene clusters using the K-means clustering approach. For the receptacle, a total of 6435 genes were found to be differentially expressed (padj < 0.01, fold change >2). All the DEGs were assigned to 12 coexpression clusters (Fig. 4b, Table S9). The expression pattern of cluster 10 exhibited a gradual decrease during receptacle development; this cluster was overrepresented with genes with GO terms associated with photosynthesis, generation of precursor metabolites, and energy-producing molecules (Table S10). Cluster 6, which consists of genes abundantly expressed in the red stage receptacle, was characterized by an abundance of genes associated with GO terms associated with catalytic activity. Many fruit ripening-related genes were in cluster 6. For example, FaMYB10 (FxaC_2g30690), predominantly expressed in the red receptacle (RR), is a master transcriptional regulator of genes acting in the flavonoid/phenylpropanoid pathway during fruit ripening32,33. Finally, we clustered the genes differentially expressed during achene development (Fig. S1, Tables S11, S12). From green achenes (GAs) to white achenes (WAs), the expression levels of 4480 genes were significantly upregulated in WAs. Among these DEGs are 23 CRU1 genes, homologs of AtCRU1 (AT5G44120), which code for a 12S seed storage protein whose phosphorylation state is modulated in response to ABA in Arabidopsis thaliana seeds34,35. However, only 25 CRU1 genes exist in F. ananassa, indicating that most CRU1 genes are highly expressed in achenes after the white stage (Fig. 4c). This indicates that achenes, which contain seeds, accumulate storage proteins after the white stage.

Strawberry genome database (SGD) based on the ViroBLAST webserver

We constructed a SGD website for our new annotations (both F. ananassa (v1.0.a2) and F. vesca (v4.0.a2)), providing easy access to the F. ananassa and F. vesca genomes through downloading and homolog searching ( (Fig. 5). The SGD website, which is based on the ViroBLAST webserver36, allows users to search (via BLAST) the cDNA and protein sequences of both the F. vesca and F. ananassa genomes. Users can upload sequences as FASTA files or paste them into the query box directly. Additionally, the website provides more BLAST options for advanced users to obtain more specific information.

Fig. 5: Image of the strawberry genome database website.

Users can perform (batch) sequence searches against F. vesca and F. ananassa nucleotide and protein databases and can download annotation files. Users can also configure the advanced BLAST parameters

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