Latest Research

Rapamycin production and growth of the SRMK07 strain The rapamycin-overproducing mutant, S. rapamycinicus SRMK07, produced approximately 207 mg/L rapamycin, or around fourfold more rapamycin than the NRRL 5491 strain (Fig. 1a). Despite the significant improvement in the rapamycin production, the SRMK07 strain showed almost normal growth in comparison to the wild-type (Fig. 1b). The wild-type and the SRMK07
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Governments around the world are redefining dual-use and critical technologies with reference to the politics of the moment, economic competitiveness, and the current distributions of global power. It has never been more important for synthetic biology practitioners to achieve coherence in the language they use in presenting the discipline to policymakers at the national and
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Molecular cloning, mutagenesis, and screening The mBlueberry2 gene was synthesized de novo by Tsingke Biotechnology Co., Ltd. based on the sequence reported in the original publication25. Plasmids encoding mScarlet, EBFP2, and mTagBFP2 were acquired from Addgene. Synthetic DNA oligonucleotides used for cloning were purchased from Tsingke Biotechnology Co., Ltd. PrimeStar Max master mix (Clontech) was
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Immune reactive tumor organoids for examining the effects of bacterial metabolites on ICB response There are components in a tumor model system required to enable adaptive anti-tumor immune response. First, a viable immune cell population, such as cytotoxic T-lymphocytes (CTLs), that can react with the specific antigens presented by the cancer cells must be present.
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Evaluation of several CBE variants recognizing NGN PAM in zebrafish Base editing requires the presence of a PAM at 13–19 bp downstream of the targeted C base. This limitation is critical and often makes CBEs unable to introduce the desired point mutation in the genome. To overcome this limitation particularly important when trying to model disease-causing
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Ethical statement Blood samples from consenting healthy donors were collected at Georgia Institute of Technology. The study included 7 healthy participants (5 male, 2 female, age range 24 to 37). The blood samples from prostate cancer patients were collected at Emory University Hospital and Grady Memorial Hospital, and the blood and ascites samples of ovarian
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Re-cutting after successful base-pair substitution As a reporter for single base-pair substitution, we used mouse Embryonic Stem Cells (mESCs) carrying a single-copy sequence that encodes green fluorescent protein (GFP) but lacks a start codon. However, an in frame AAG triplet is present, which allows for GFP expression upon conversion into ATG (Fig. 1A)26. Figure 1 Re-cutting
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Measurement automation, platform software integration, and reactive experiment control with ReacSight The ReacSight strategy to enhance bioreactor arrays for automated measurements and reactive experiment control combines hardware and software elements in a flexible and standardized manner (Fig. 1, Supplementary Note 1). A pipetting robot is used to establish, in a generic fashion, a physical link between any
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Synthesis of L-biotin derivatives General Unless otherwise noted, all reactions were performed in heat gun dried glassware, sealed with a rubber septum under an atmosphere of nitrogen or argon. Materials were obtained from suppliers and used without further purification. Flash column chromatography was carried out using Cica Silica Gel 60 N (spherical/40–50 μm). Reactions and chromatography fractions
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Preparation of A. salmonicida Atypical A. salmonicida (As) (strain B10F21, T1031) was cultured on heart infusion agar (HI; Nissui Pharmaceutical Co., Ltd., Tokyo, Japan) plates for 72 h at 20 °C. The colonies were identified as As using flow cytometry (FCM) using anti-A. salmonicida (anti-As) monoclonal antibody (MAb)17. The selected colonies were sub-cultured in 500-ml HI broth
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Mice NOD/LtJ (JAX# 001976), C57BL/6 (B6) (JAX# 000664), NOD.c3c4 (JAX# 010971), and B6.Cxcr6Gfp (JAX# 005693) mice were purchased from the Jackson Laboratory (Bar Harbor, ME). 8.3-NOD.G6pc2–/–.Tcra–/– mice have been described68. B6.Ifng-ARE-Del−/− mice were obtained from H. Young (NIH, Bethesda, MD)26. NOD.c3c4.scid mice were generated by backcrossing (NOD.c3c4 x NOD.scid) F1 mice with NOD.c3c4 mice for
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Materials β-nicotinamide adenine dinucleotide (NAD+), reduced β-nicotinamide adenine dinucleotide (NADH), calcium chloride (CaCl2), disodium hydrogen phosphate (Na2HPO4), zinc nitrate hexahydrate, 2-methylimidazole and LPS (from Escherichia coli O111:B4) were purchased from Sigma-Aldrich. Soy PC and DOPA were purchased from Avanti Polar Lipids. Other reagents and solvents were purchased from Thermo Fisher Scientific and used as received
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Construction of CO/formate bioconversion mutants via molecular fusion of two Fe-S proteins The redox proteins CODH and FDH from T. onnrineus NA1 were systematically engineered through molecular fusion of electron-transferring Fe-S proteins to construct a single redox complex. The codh and fdh3 gene clusters included the codhABCD and focA-fdh3ABC operons, respectively26,30 (Supplementary Fig. 1a and Supplementary
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Figure 2 gives an overview of the process of sweat monitoring, including sweat secretion, collection, sensing, transmission, and analysis. In all parts of the process, researchers are concerned with components in sweat that can be detected, how to achieve accurate monitoring with a small sample volume and how to design sensitive sensor devices. The question
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Natural substances and their derivatives might have prospective biological properties for oral biofilms modulation and can potentially be applied to control either root or dentinal caries. The tested compounds were synthesized from CNSL, which the components are rich in phenolic lipids, including AA and CD mixtures. The AA-derivative compound LDT11 has shown promising multifunctional biological
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Aptamers are expected to be next-generation drugs, but identifying candidate aptamers is a challenging task given the large search space. Now, an artificial intelligence (AI)-powered tool called RaptGen is proposed for improving the successful identification of aptamer sequences. Aptamers are molecular biosensors that can ‘sense’ specific target molecules. These molecular biosensors have a wide range
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Type I porcine atelocollagen was purchased from Nippon Meat Packers Inc. (Tokyo, Japan). PyKC was synthesized according to a previously published report21. All reagents were of analytical grade and used as received. All chemicals were procured from Sigma-Aldrich (St. Louis, MO) if not mentioned otherwise. Fabrication of collagen hydrogel Control collagen hydrogels were made following
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Base-editing as a feasible tool to introduce genome-wide functional knock-outs in Pseudomonas A CBE converts cytidine into thymine (C·G → T·A) in target regions of the genome (Fig. 1A). This alteration, ultimately fixed in the genome during DNA repair and replication (Fig. 1B), can be harnessed to introduce premature STOP codons in open reading frames (ORFs) that lead to
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