Latest Research

Experimental design This study aimed to develop an ex vivo 3D organotypic model of the epicardial/myocardial interface, which would enable studies directed at identifying mechanisms of adult epicardium reactivation. Our experiments verified the maintenance of the tissue architecture in the slices and the preservation of a living and healthy epicardial cells monolayer and myocardial tissue.
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We have here conducted a comparative analysis using two NGS platforms to clarify the structure of the sSMC in two different patients. We first performed WGS using an Illumina short-read sequencer. Without enrichment, 99% of all the sequence reads were mapped to an off-target region and thus wasted. To avoid this, we applied adaptive sampling,
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Materials Recombinant human Met ectodomain-Fc, rhEPOR ectodomain-Fc, and rhHGF were purchased from R&D Systems (Minneapolis, MN, USA). rhEPO and rhTPO were purchased from Peprotech, Inc. (Rocky Hill, NJ, USA). Monomeric macrocyclic peptides (aMD4dY and EMP35) were synthesized by GenScript Japan (Tokyo, Japan). Ammonium acetate was purchased from Nacalai Tesque (Kyoto, Japan). Thermolysin and ProAlanase were
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Study design AAT was produced by decellularizing adipose tissue using a combination of mechanical and chemical processing steps, and the material was characterized to determine physical and biochemical properties. In vitro studies evaluated adipogenesis and migration of cells in response to AAT, while in vivo biocompatibility, volume retention, and local immune cell populations infiltrating subcutaneously
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Determination of CD44 and EpCAM antigen levels in MCF7 cells We examined the labeling efficiency of MCF7 cells, the most common breast cancer cells, with anti-CD44 ab and anti-EpCAM ab43. Briefly, cultured MCF7 cells were labeled by incubation with Syto-13 nucleus-staining dye, QD585-anti-CD44 ab, and QD655-anti-EpCAM ab. The details of the cell culture condition, ab
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Generation of knock-down resistance alleles of the voltage-gated sodium ion channel in Drosophila Pyrethroids and DDT bind to the voltage-gated sodium channels (VGSCs) triggering abnormal channel activity but are unable to bind or disrupt function of Knockdown-resistant (Kdr) mutant channels. The most widespread kdr alleles in Anophelines include L1014F, whereas I1011M/V are prevalent in Aedes
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Plasmid construction Sequence of the affinity clamp (PDZ-fibronectin fusion protein) was extracted from the PDB file 3CH8. Sequences of the anti-HSA scFv and Fab antibody fragments were derived from the PDB file 5FUO or from the US_2014_0186365 patent (sequence 4D2). Sequences of the anti-IL-6 scFv, anti-IL-6 Fab, anti-IFNα-1b scFv, and anti-IFNα-2a Fab were derived from the following
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Pre-clinical animal study design This study enrolled 15 Yucatan minipigs ranging from 40 to 50 days of age that were assigned to three cohorts with different survival time points (Cohort 1; 365 ± 15 days, Cohort 2; 90 ± 3 days and Cohort 3; 30 ± 3 days post-implantation. American Preclinical Sciences, Minneapolis, MN IACUC protocols OIP007-IS75/OIP010-IS75). The number of animals,
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The blood–brain barrier (BBB) constitutes the main access point into the brain parenchyma for endogenous and therapeutic molecules alike. Molecular transport across the BBB is highly regulated, owing principally to its unique cellular architecture with endothelial cells (ECs) lining the blood vessels, pericytes (PCs) in close contact with the ECs, and astrocytes (ACs) extending their
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Optimization of microwave-alkaline hydrolysis The nutrient analysis of both DSM and JP, as well as molasses, were broken down into several main nutritional components as shown in Table 1. More than 50% nutrient fraction of DSM consisted of protein while more than two-thirds of JP comprised of carbohydrates. Both substrates are excellent nitrogen and carbon
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Study design and samples details Research protocols were approved and monitored by the USDA, ARS, U.S. Meat Animal Research Center Institutional and Animal Care Committee in accordance with the Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching (2010). All authors complied with the ARRIVE guidelines. Calves (n = 75) used in
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Materials and reagents Gelatin from porcine skin (type A) was purchased from Sigma-Aldrich (St. Louis, MO) and used as received. Amine-terminated G5 PAMAM dendrimers were purchased from Dendritech (Midland, MI). Sodium periodate, dicyclohexylcarbodiimide, and N-hydroxysuccinimide were purchased from Macklin Reagent Co. (Shanghai, China). Adipic dihydrazide and Lipofectamine 3000 were obtained from Invitrogen (Carlsbad, California). MiR-140
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The emergence of nanopore sequencing is reshaping the landscape of genomics. Devices from Oxford Nanopore Technologies (ONT) enable sequencing of native DNA and RNA molecules with no theoretical upper limit on read length1. This supports the accurate assembly and phasing of repetitive genomes and metagenomes2,3,4,5,6; enhanced resolution of structural variation7,8,9,10,11 and spliced RNA transcripts12; and
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Development of a systematic evolution platform We began by developing a 96-well plate-based method, wherein 500-µl cultures of evolving M13 bacteriophage (Fig. 1a) are serially diluted with fresh host bacteria twice per hour using an automated liquid handler (Fig. 1b). To enable the pipetting speeds required to approximate continuous flow, we developed a robotic Python
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Clinical benefits of combining H&E and IHC on the same slide include conservation of small specimens, such as biopsies, to maximize the amount of clinical information obtained from the specimen, and the ability to evaluate protein expression within the full H&E context, bringing to bear the trove of information that H&E based staining provides the
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Data summary and genomic distribution of SNPs After filtering the datasets to keep only fungal species represented by both SNP and transcriptome information, we kept five common phytopathogenic fungi: Cadophora gregata, Fusarium graminearum, Fusarium virguliforme, Macrophomina phaseolina, and Phakopsora pachyrhizi (Fig. 1A). Overall, SNPs were located in gene-rich regions of the genome (Fig. 1B). SNPs were unevenly
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Identification of improved Gal2 mutants To identify Gal2 variants with improved xylose transport properties in the presence of glucose, the GAL2 wildtype open reading frame was subjected to error-prone PCR (epPCR) as described in “Materials and methods”. The obtained PCR products were co-transformed with the linearized p426H7 plasmids into the strain AFY10X to allow for
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Design and synthesis of oligonucleotides A series of 16mer ASOs were designed to target mouse Itga4 and Dmpk mRNA, Malat1 RNA, and human Stat3 mRNA. The ASOs had a 10 DNA nucleotide gapmer structure flanked by 3 LNA nucleotides. All internucleotide linkages were modified by phosphorothioate substitution to increase plasma ASO stability and protein binding,
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Training in visualization should be a part of every scientist’s journey. In January 2020, the Centers for Disease Control and Prevention (CDC) released an image that took the world by storm: that of the SARS-CoV-2 virus. This image finally gave a face to an invisible killer that was tearing through the city of Wuhan, China,
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Pancreatic ductal adenocarcinoma (PDA) is a deadly disease where both cancer cells and host pancreatic cells in the tumour microenvironment play key roles in cancer progression. Organoids are three-dimensional multicellular structures that can self-organize into physiologically relevant morphologies, and can be used to study complex cellular networks in disease models and identify potential therapeutic targets.
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